DNA Mismatch Detection by Metal Ion Enhanced Impedance Analysis

نویسندگان

  • Peng-Chung Jangjian
  • Tzeng-Feng Liu
  • Chuan-Mei Tsai
  • Mei-Yi Li
  • Ming-Shih Tsai
  • Shin-Hua Tseng
  • Tsai-Mu Cheng
  • Chia-Ching Chang
  • PENG-CHUNG JANGJIAN
  • TZENG-FENG LIU
چکیده

Peng-Chung Jangjian, Tzeng-Feng Liu, Chuan-Mei Tsai, Mei-Yi Li, 2 Ming-Shih Tsai, Shin-Hua Tseng, Tsai-Mu Cheng, and Chia-Ching Chang 5, ∗ Department of Material Science and Engineering, National Chiao Tung University, Hsinchu, 30050, Taiwan National Nano Device Laboratories, Hsinchu, 30072, Taiwan Cabot Microelectronic, Chupei, Hsinchu Hsien, 302, Taiwan Department of Biological Science and Technology, National Chiao Tung University, Hsinchu, 30050, Taiwan Institute of Physics, Academia Sinica, Taipei, 11529, Taiwan (Received May 12, 2009)

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Shear and AC Field Enhanced Carbon Nanotube Impedance Assay for Rapid, Sensitive, and Mismatch-Discriminating DNA Hybridization.

Other than concentrating the target molecules at the sensor location, we demonstrate two distinct new advantages of an open-flow impedance-sensing platform for DNA hybridization on carbon nanotube (CNT) surface in the presence of a high-frequency AC electric field. The shear-enhanced DNA and ion transport rate to the CNT surface decouples the parasitic double-layer AC impedance signal from the ...

متن کامل

Interaction of metal ions and DNA films on gold surfaces: an electrochemical impedance study.

Electrochemical impedance spectroscopy (EIS) has been used to investigate the effects of a number of metal ions with DNA films on gold surfaces exploiting [Fe(CN)6](3-/4-) as a solution-based redox probe. Alkaline earth metal ions Mg2+, Ca2+, trivalent Al3+, La3+ and divalent transition metal ions Ni2+, Cu2+, Cd2+ and Hg2+ have been selected in this study and the results are compared with previ...

متن کامل

Designing DNA interstrand lock for locus-specific methylation detection in a nanopore

DNA methylation is an important epigenetic regulation of gene transcription. Locus-specific DNA methylation can be used as biomarkers in various diseases including cancer. Many methods have been developed for genome-wide methylation analysis, but molecular diagnotics needs simple tools to determine methylation states at individual CpG sites in a gene fragment. In this report, we utilized the na...

متن کامل

Electrochemical detection of single-nucleotide mismatches: application of M-DNA.

The detection of a single-nucleotide mismatch in unlabeled duplex DNA by electrochemical methods is presented. Impedance spectroscopy is used to characterize a perfect duplex monolayer and three DNA monolayers differing in the position of the mismatch. The monolayers were studied as B-DNA (normal duplex DNA) and after conversion to M-DNA (a metalated duplex). Modeling of the impedance data to a...

متن کامل

Fluorescence Chemosensing of Mg2+ by Phenylhydrazone of a Difluorenylpiperidin-4-one

Magnesium is an abundant element in the environment. Magnesium ion sensing by fluorescence spectral method is of importance due to the need for the detection of the metal in the human body and the environment. In this paper, we report the Mg2+ ion sensing behavior of the phenylhydrazone derivative of a difluorenylpiperidin-4-one. The preparation method of this compound is simple. The compound s...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

برای دانلود متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

ثبت نام

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

عنوان ژورنال:

دوره   شماره 

صفحات  -

تاریخ انتشار 2009